Rakukultuur

Raku vananemine. Mikrokoloonia kasvamise 305 minutit on kiirendatud 7 sekundiks. Algsest ühest rakust moodustub 505 rakku.

Rakukultuur on toimingute kogum, mis võimaldab kasvatada elusorganismide rakke väljaspool organismi ehk in vitro. Tüüpiliselt kasvatatakse inimese eri kudedest eraldatud rakke, kuid on üsna levinud ka rakud muudest organismidest, sh imetajad (nt hiina hamster, rohepärdik, kodukoer) kui loomad üldisemalt (nt uitöölane). Taime-, seene- ja bakterirakkude kasvatamise kohta kasutatakse enamasti täpsustavaid mõisteid (nt bakterikultuur).^[1]^[2]^[3]

Enamasti kasvavad ühes kultuuris ühte tüüpi rakud, nii algse elundi (nt kopsud, nahk) kui ka rakutüübi (nt fibroblastid või epiteelirakud) mõistes. Samas kuulub rakukultuuri arengusuundade hulka ka rakkude nn kooskultiveerimine (inglise keeles co-culturing).^[4] Rakukultuur loob väärtuslikke mudelsüsteeme, milles on võimalik läbi viia teaduslikke uuringuid: näiteks mõõta ravimikandidaatide omadusi,^[5]^[6]^[7] teha kindlaks teatud valgu rolli elusrakus,^[8]^[9] jäljendada füsioloogilisi protsesse^[10]^[11] või toota terapeutilisi antikehi või vaktsiine.^[12]^[13]

↑ Victor M. Loyola-Vargas, Felipe Vázquez-Flota (2006). "An Introduction to Plant Cell Culture". An Introduction to Plant Cell Culture (Book series Methods in Molecular Biology). Springer Link. Lk 3–8.
↑ Andrés Garzón, Juan Jimenez (2015). Yeast Cell Culture. eLS: Wiley.
↑ A Rouf, Varsha Kanojia, HR Naik, Bazilla Naseer, Tahiya Qadri (2017). An overview of microbial cell culture. Journal of Pharmacognosy and Phytochemistry: Society of Pharmacognosy and Phytochemistry. Lk 1923–1928.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)
↑ Michelle A. M. Vis, Keita Ito, Sandra Hofmann (2020). Impact of Culture Medium on Cellular Interactions in in vitro Co-culture Systems. Frontiers in Bioengineering and Biotechnology: Frontiers.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)
↑ Ye Fang, Richard M. Eglen (2017). Three-Dimensional Cell Cultures in Drug Discovery and Development. SLAS Discovery: Elsevier. Lk 456–472.
↑ Maris-Johanna Tahk, Jane Torp, Mohammed A S Ali, Dmytro Fishman, Leopold Parts, Lukas Grätz, Christoph Müller, Max Keller, Santa Veiksina, Tõnis Laasfeld, Ago Rinken (2022). Live-cell microscopy or fluorescence anisotropy with budded baculoviruses—which way to go with measuring ligand binding to M4 muscarinic receptors?. Open Biology: The Royal Society.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)
↑ Viitamistõrge: Vigane <ref>-silt. Viide nimega :3 on ilma tekstita.
↑ Sergei Kopanchuk, Edijs Vavers, Santa Veiksina, Kadri Ligi, Liga Zvejniece, Maija Dambrova, Ago Rinken (2022). Intracellular dynamics of the Sigma-1 receptor observed with super-resolution imaging microscopy. PLoS One: Public Library of Science.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)
↑ Jurgita Matulienė, Gediminas Žvinys, Vytautas Petrauskas, Agnė Kvietkauskaitė, Audrius Zakšauskas, Kirill Shubin, Asta Zubrienė, Lina Baranauskienė, Lina Kačenauskaitė, Sergei Kopanchuk, Santa Veiksina, Vaida Paketurytė-Latvė, Joana Smirnovienė, Vaida Juozapaitienė, Aurelija Mickevičiūtė, Vilma Michailovienė, Jelena Jachno, Dovilė Stravinskienė, Aistė Sližienė, Agnė Petrošiūtė, Holger M Becker, Justina Kazokaitė-Adomaitienė, Ala Yaromina, Edita Čapkauskaitė, Ago Rinken, Virginija Dudutienė, Ludwig J Dubois, Daumantas Matulis (2022). Picomolar fluorescent probes for compound affinity determination to carbonic anhydrase IX expressed in live cancer cells. Scientific Reports: Nature.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)
↑ Jean Livet, Tamily A Weissman, Hyuno Kang, Ryan W Draft, Ju Lu, Robyn A Bennis, Joshua R Sanes, Jeff W Lichtman (2007). Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature: Nature. Lk 56–62.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)
↑ Viitamistõrge: Vigane <ref>-silt. Viide nimega :5 on ilma tekstita.
↑ Cary Matanguihan, Paul Wu (2022). Upstream continuous processing: recent advances in production of biopharmaceuticals and challenges in manufacturing. Current Opinion in Biotechnology: Elsevier.
↑ S Furkan Demirden, Kadir Alptekin, Ilgin Kimiz-Gebologlu, Suphi S Oncel (2022). Influenza Vaccine: An Engineering Vision from Virological Importance to Production. Biotechnology and Bioprocess Engineering: Springer Link. Lk 1–25.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)

[1] Victor M. Loyola-Vargas, Felipe Vázquez-Flota (2006). "An Introduction to Plant Cell Culture". An Introduction to Plant Cell Culture (Book series Methods in Molecular Biology). Springer Link. Lk 3–8.

[2] Andrés Garzón, Juan Jimenez (2015). Yeast Cell Culture. eLS: Wiley.

[3] A Rouf, Varsha Kanojia, HR Naik, Bazilla Naseer, Tahiya Qadri (2017). An overview of microbial cell culture. Journal of Pharmacognosy and Phytochemistry: Society of Pharmacognosy and Phytochemistry. Lk 1923–1928.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)

[:9-4] Michelle A. M. Vis, Keita Ito, Sandra Hofmann (2020). Impact of Culture Medium on Cellular Interactions in in vitro Co-culture Systems. Frontiers in Bioengineering and Biotechnology: Frontiers.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)

[5] Ye Fang, Richard M. Eglen (2017). Three-Dimensional Cell Cultures in Drug Discovery and Development. SLAS Discovery: Elsevier. Lk 456–472.

[6] Maris-Johanna Tahk, Jane Torp, Mohammed A S Ali, Dmytro Fishman, Leopold Parts, Lukas Grätz, Christoph Müller, Max Keller, Santa Veiksina, Tõnis Laasfeld, Ago Rinken (2022). Live-cell microscopy or fluorescence anisotropy with budded baculoviruses—which way to go with measuring ligand binding to M4 muscarinic receptors?. Open Biology: The Royal Society.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)

[:3-7] Viitamistõrge: Vigane <ref>-silt. Viide nimega :3 on ilma tekstita.

[8] Sergei Kopanchuk, Edijs Vavers, Santa Veiksina, Kadri Ligi, Liga Zvejniece, Maija Dambrova, Ago Rinken (2022). Intracellular dynamics of the Sigma-1 receptor observed with super-resolution imaging microscopy. PLoS One: Public Library of Science.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)

[9] Jurgita Matulienė, Gediminas Žvinys, Vytautas Petrauskas, Agnė Kvietkauskaitė, Audrius Zakšauskas, Kirill Shubin, Asta Zubrienė, Lina Baranauskienė, Lina Kačenauskaitė, Sergei Kopanchuk, Santa Veiksina, Vaida Paketurytė-Latvė, Joana Smirnovienė, Vaida Juozapaitienė, Aurelija Mickevičiūtė, Vilma Michailovienė, Jelena Jachno, Dovilė Stravinskienė, Aistė Sližienė, Agnė Petrošiūtė, Holger M Becker, Justina Kazokaitė-Adomaitienė, Ala Yaromina, Edita Čapkauskaitė, Ago Rinken, Virginija Dudutienė, Ludwig J Dubois, Daumantas Matulis (2022). Picomolar fluorescent probes for compound affinity determination to carbonic anhydrase IX expressed in live cancer cells. Scientific Reports: Nature.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)

[10] Jean Livet, Tamily A Weissman, Hyuno Kang, Ryan W Draft, Ju Lu, Robyn A Bennis, Joshua R Sanes, Jeff W Lichtman (2007). Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature: Nature. Lk 56–62.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)

[:5-11] Viitamistõrge: Vigane <ref>-silt. Viide nimega :5 on ilma tekstita.

[12] Cary Matanguihan, Paul Wu (2022). Upstream continuous processing: recent advances in production of biopharmaceuticals and challenges in manufacturing. Current Opinion in Biotechnology: Elsevier.

[13] S Furkan Demirden, Kadir Alptekin, Ilgin Kimiz-Gebologlu, Suphi S Oncel (2022). Influenza Vaccine: An Engineering Vision from Virological Importance to Production. Biotechnology and Bioprocess Engineering: Springer Link. Lk 1–25.{{raamatuviide}}: CS1 hooldus: mitu nime: autorite loend (link)

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